Tag

Part:BBa_K4375008:Design

Designed by: Zska Csorba   Group: iGEM22_ELTE   (2022-09-28)


miRFP670 nano


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 52
    Illegal PstI site found at 310
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 52
    Illegal PstI site found at 310
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 52
    Illegal PstI site found at 310
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 52
    Illegal PstI site found at 310
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was codon optimised for production in E.coli


Source

The sequence was described in: Oliinyk, O.S., Shemetov, A.A., Pletnev, S. et al. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun 10, 279 (2019). https://doi.org/10.1038/s41467-018-08050-8

References